Saccharification of pineapple peelings through dilute acid mhydrolysis

Cotton plugs and aluminium foils were put in the containers and autoclaved at oC for 15 minutes. This was done to comply with a standard that will consequently referred for determining the cell number per mL.

The graph of the predicted against the actual shown below also confirms that the prediction was accurate. This is an Open Access article distributed under the following Assignment of Rights http: Due to its high cellulosic contents a large number of applications is under study such as manure Ultra et al.

This was shown in Table 5 where the p-values are greater than 0.

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The rate of the glucose decrease above the optimum value of concentration was low. The adequacy of the model can also be seen in Figure 8 where the actual points lie along the diagonal. According to Drapcho et. The effects of four physical pre-treatments were investigated to reduce biomass recalcitrance.

In order to obtain the growth rate of specific yeast, standardization must be done. Transportation roughly uses 30 percent energy usage and about 95 percent of the global oil consumption [4]. Due to its limited respiratory system that has the ability of a Crabtree effect, the yield loss during the conversion occurs in little amounts only.

Alcohol was determined using Gas Chromatography. This is due to the usage of substrate unaccompanied from respiration. The bottles were cooled to room temperature to prevent the yeast degradation.

Bioethanol Production from Pineapple (Ananas ...

Abstract This work estimates the potential of banana pseudo-stem with high cellulosic content The design matrix with eighteen experimental runs in two blocks with four replicates of the midpoint was used.

The closeness of R2 to 1 also confirmed that the kinetics of the fermentation was described by the Michaelis- Menten equation. The contour and 3D plots of substrate concentration against temperature. Tokyo Japan at different temperatures oC at pressure 0. After inoculation, the broth containing the yeast was serially diluted with NSS up to its 10th dilution.

The contour and 3D plots of time against temperature. Nine 9 mL of NSS was transferred into each of the 10 test tubes. Fruit waste contains reusable substances of high value and potential use. The objectives of this study were to: The adjustment of pH was done with 1.

The Michaelis-Menten Model Following the linear form of model equation Equation 7 7 The kinetics parameters and the fitness were determined using the linear plot shown in Figure The bottles were cooled to room temperature to prevent the yeast degradation. The goodness of fit of the model was checked by the determination coefficient R2.

The interactive effects of the pH with other factors were not significant. Ethanol extractive covers a great amount of dry pineapple waste at The contour and 3D plots of pH against temperature.

The pretreatment uses a number of techniques including biological treatment, and steam explosion to alter the structure of cellulosic biomass to make cellulose more accessible, such that enzymes digest the cellulosic biomass completely for production of sugars Parveen et al.

It has been a growing interest for many years because of its alternative green energy sources, consequently minimizing greenhouse gas GHG emission and finally help alleviate the rise of fuel prices []. The dried powdered material was then stored at room temperature away from sunlight.In fact, dilute acid hydrolysis of OPW up to °C was reported to be unable to hydrolyze pectin to galacturonic acid, whereas an ACSE pretreatment at °C for 6 min yielded as much as % of pectin solubilization.

Thus, such solubilization yields should. This study aims to saccharify pineapple Ananas comosus peelings through dilute acid hydrolysis. Specifically, the study attempts: 1. To know whether the dilute acid hydrolysis is an effective way of yielding concentration of sugar from pineapple peelings; 2.

To determine whether the temperature has an effect on the concentration of sugar; 3. Enzymatic hydrolysis of the peel and core wetted samples was carried out after each pre-treatment in a pH range from to by adding 6 g/kg fw of cellulase, 1 g/kg fw of hemicellulase, and g/kg fw of pectinase at 50 °C for 24 h, using the proposed optimal conditions for pineapple waste hydrolysis according to previous studies (De Prados et.

Three different fermentation modes, direct fermentation, separate hydrolysis and fermentation, and simultaneous saccharification and fermentation of the biomass were tested and compared. The results show that the main sugars obtained from pineapple waste were: glucose, uronic acid, xylose, galactose, arabinose and mannose.

Enzymatic hydrolysis of sulphuric acid treated biomass was % and % when pretreatment was done at 90 °C and °C at same acid concentration, while sulfide shows % and % hydrolysis yield respectively. Pineapple Peel Hydrolysis by T. viride Crude Extract Passion fruit peel was treated with 1 M NaOH, autoclaved at °C for 30 min and washed with distilled water.

The pH was then neutralized with dilute phosphoric acid and the mixture was dried at 70 °C [ 30 ].

Saccharification of pineapple peelings through dilute acid mhydrolysis
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